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anti-HTRA2 antibody product blog

Posted on 2017-05-23 11:25:40 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; HTRA2; anti-HTRA2 antibody;
The HTRA2 htra2 (Catalog #MBS9231146) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-HTRA2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s HTRA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP).
WB: 1:500. Researchers should empirically determine the suitability of the HTRA2 htra2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The HTRA2 htra2 product has the following accession number(s) (GI #1012282649) (NCBI Accession #NP_001308656.1) (Uniprot Accession #O43464). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-HTRA2 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot detection of HtrA2/Omi in 293T cell lysates using HtrA2/Omi mouse mAb (dilution 1:500).Predicted band size:49kDa.Observed band size:36kDa.)
Western Blot (WB) HTRA2.

Immunoprecipitation (IP) (Immunoprecipitation analysis of Hela cell lysates using HtrA2/Omi mouse mAb.)
Immunoprecipitation (IP) HTRA2.

Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.

Cellular Location: Mitochondrion intermembrane space. Mitochondrion membrane; Single-pass membrane protein. Note=Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID
Tissue Location: Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HTRA2 are readily searchable from our website. Different antibodies against the same target such as HTRA2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Brain, Embryonic Tissue, Eye, Kidney, Lung, Prostate, Skin, Stomach tissues are correlated with this protein. HTRA2 also interacts with the following gene(s): BIRC2, BIRC6, DIABLO, LATS1, PINK1, UBC, XIAP. The following patways have been known to be associated with this gene.
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