anti-ICOS antibody product blog
Tags: Antibody; Monoclonal Antibody; ICOS; anti-ICOS antibody;
The ICOS n/a (Catalog #MBS834882) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s ICOS can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).FC: <= 0.5 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the ICOS n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ICOS antibody (biotin) with the following immunoassay(s):
Flow Cytometry (Flow Cytometric analysis using ICOS antibody
Staining of unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.03 ug of Rat IgG2b Isotype Control (biotin) (open histogram) or 0.03 ug of CD278 antibody (biotin) followed by Streptavidin (PE). Viable cells were analyzed.)
Rat monoclonal ICOS antibody (biotin).
Biological Significance: The 7E.17G9 monoclonal antibody reacts with mouse ICOS, a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells. Tag/Conjugate: Biotin. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ICOS are readily searchable from our website. Different antibodies against the same target such as ICOS may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.