anti-ICT1 antibody product blog
Tags: Antibody; Monoclonal Antibody; ICT1; anti-ICT1 antibody;
The ICT1 mrpl58 (Catalog #MBS200279) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ICT1 antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s ICT1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS).Recommended starting dilution is 1:1000. Researchers should empirically determine the suitability of the ICT1 mrpl58 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ICT1 mrpl58 product has the following accession number(s) (GI #4557657) (NCBI Accession #NP_001536) (Uniprot Accession #Q14197). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ICT1 antibody with the following immunoassay(s):
Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ICT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ICT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: ICT Transfected 293T cell lysate)
Peptidyl-tRNA hydrolase ICT1 acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. This neoplastic-induced deviation is marked by a change in expression of several mRNAs. ICT1 is a member of the prokaryotic/mitochondrial release factor family whose expression is downregulated over 4 fold upon colon stem cell differentiation. This downregulation of ICT1 could lead to its use as a marker for detection of colon carcinomas.
Antigen Species: Human
Immunogen: Recombinant human ICT1 (30-206aa) purified from E. coli. Clone Note: Anti-human ICT1 mAb, clone AT1E9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ICT1 protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ICT1 are readily searchable from our website. Different antibodies against the same target such as ICT1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.