anti-IFN gamma antibody product blog
Tags: Antibody; Monoclonal Antibody; IFN gamma; anti-IFN gamma antibody;
The IFN gamma ifng (Catalog #MBS666497) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human IFN gamma -FITC reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s IFN gamma can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).1:50 (or use 1.6ml of concentrated stock per 5 x 105-cell test). Researchers should empirically determine the suitability of the IFN gamma ifng for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IFN gamma ifng product has the following accession number(s) (GI #56786138) (NCBI Accession #NP_000610.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human IFN gamma -FITC with the following immunoassay(s):
Testing Data
Information: The cytokine human interferon-gamma (IFN-gamma) is a glycosylated protein that exists as a homodimer of 34 kd in its biologically active form. IFN-gamma is expressed upon activation by TH1 cells CD8+ cells and NK cells (2). Secreted IFN-gamma is a potent inhibitor of in vitro hematopoiesis and appears to serve as an important regulator of lymphocyte and macrophage function by exerting antiproliferative effects (1). Antibody ANC2E11 binds to recombinant and cellular IFN-gamma in EIA and Flow cytometry.
Performance: Ficoll prepared human peripheral blood lymphocytes were stimulated 4 hours in the presence of 50 ng/ml PMA, 2 muM ionomicin and 5muM monensin. Cells were then harvested, washed and fixed for 30 min with 2% buffered formaldehyde after which they were washed two times. Fixed cells were permeabolized 10 minutes in a buffer containing 0.05% Saponin. Subsequent reagent incubations and washes were done using this buffer. Five x 105 cells per tube were preincubated 5 minutes with 20 mul of 250 mug/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 mul of anti-IFN-gamma/FITC at a 1:50 dilution factor (2 mug/ml). Cells were washed three times, fixed and analyzed by FACS. A net 21.9% sub population of the cells stained positive with a mean shift of 1.2 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 mul of 0.5 mg/ml anti-IFN-gamma antibody. Conjugation: FITC
Production: Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at 0.1 mg/ml with a Fluorescein/IgG molar ratio of 8.1.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: Recombinant human IFN-gamma.