|The IFN-gamma ifng (Catalog #MBS690653) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human IFN-gamma reacts with Human and may cross-react with other species as described in the data sheet.
Western Blot: To detect hIFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIFN-gamma is 2.0-4.0 ng/lane, under non-reducing conditions. Researchers should empirically determine the suitability of the IFN-gamma ifng for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IFN-gamma ifng product has the following accession number(s) (GI #56786138) (NCBI Accession #NP_000610.2) (Uniprot Accession #P01579). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
IFN-gamma is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-gamma receptors are present in most immune cells, which respond to IFN-gamma signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-gamma signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-gamma stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-gamma species-specific and is biologically active only in human and primate cells. Recombinant human IFN-gamma is a 16.7 kDa protein containing 143 amino acid residues.
Antibody Generation: Produced in BALB/c mice using highly pure (>98%) recombinant human IFN-gamma as the immunizing antigen. This IgG1K antibody was purified from ascites fluid by antigen affinity chromatography. Buffer: PBS, pH 7.2. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IFN-gamma are readily searchable from our website. Different antibodies against the same target such as IFN-gamma may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Arthritis, Rheumatoid, Autoimmune Diseases, Diabetes Mellitus, Type 1, HIV Infections, Hepatitis, Hepatitis C, Lymphoproliferative Disorders, MYCOBACTERIUM TUBERCULOSIS, SUSCEPTIBILITY TO, Necrosis, Respiratory Tract Diseases are some of the diseases may be linked to Mouse Anti-Human IFN-gamma. Blood, Kidney, Lymph Node tissues are correlated with this protein. IFN-gamma also interacts with the following gene(s): CD4, CD86, IFNGR1, IL18, IL2, IRF1, STAT1, TBX21.