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anti-IgA antibody product blog

Posted on 2015-12-07 14:59:51 by mybiosource_staff
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Tags: Antibody; IgA; Anti-IgA Antibody; Monoclonal Antibody;
The IgA n/a (Catalog #MBS666334) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human IgA-Biotin reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s IgA can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA). Researchers should empirically determine the suitability of the IgA n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The IgA n/a product has the following accession number(s) (GI #2632187) (NCBI Accession #CAA10818.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human IgA-Biotin with the following immunoassay(s):
Testing Data
Testing Data IgA.

Information: Human immunoglobulins are glycoproteins composed of two disulfide-bonded heavy (H) chain subunits, each of which is linked by interchain disulfide bonds to a light (L) chain forming a tetramolecular complex. There are five classes of immunoglobulins, designated IgG, IgA, IgM, IgD and IgE, which are defined by differences in the constant region of H chains. L chains are divided into kappa or lambda classifications based on structural antigenic differences. All classes of immunoglobulins have been found on the cell surface of B lymphocytes where they function as antigen receptors to elicit antigen-dependent proliferation and secretion of antigen specific soluble circulating antibodies. Antibody Hisa43 recognizes cell surface expressed IgA.

Performance: Five x 105 ficoll prepared human peripheral white blood cells were pre incubated 5 minutes with 20 ul of 250 ug/ml human Ig after which they were incubated 45 minutes on ice with 80 ul of anti-human IgA/Biotin at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed by FACS. A net 8.6% sub population of the cells stained positive with a mean shift of 1.22 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-IgA antibody. Conjugation: Biotin
Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: Human salivary proteins. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IgA are readily searchable from our website. Different antibodies against the same target such as IgA may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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