anti-IGF-1 Clone 1F-3H10 antibody product blog
Tags: Antibody; Monoclonal Antibody; IGF-1 Clone 1F-3H10; anti-IGF-1 Clone 1F-3H10 antibody;
The IGF-1 Clone 1F-3H10 n/a (Catalog #MBS350013) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-human Insulin-like Growth Factor 1 Monoclonal Antibody reacts with HumanCrossreactivity Note: No cross reaction with insulin or IGF-2 and may cross-react with other species as described in the data sheet. MyBioSource\'s IGF-1 Clone 1F-3H10 can be used in a range of immunoassay formats including, but not limited to, Indirect ELISA, Western Immunoblot, Western Blot, Immunofluorescent Staining.
ELISA: This monoclonal antibody containing ascites fluid has been found by indirect ELISA to bind to hIGF-1. and titration experiments show the titer to be greater than 1:5000.
Western Blot: Western immunoblots resulted in a single band being detected at ~ 7.5 kDa at a dilution of 1:5000
Immunofluorescent: This monoclonal antibody has been found to stain cells which produce IGF-1 at a 1:2000 dilution. The cultured cells were washed x 4 and fixed with neutral buffered formalin
Immunofluorescent: This monoclonal antibody has been found to stain cells which produce IGF-1 at a 1:2000 dilution. The cultured cells were washed x 4 and fixed with neutral buffered formalin
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the mouse ascites after dilution to at least 1:5000 (Note: higher dilutions may be needed). Use 1% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the antibody bind for 1 - 2 hours.
4. Wash x 4 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-mouse IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 1% normal goat serum in TBS/Tween-20. Incubate 1 - 2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 6 for at least 5 min per wash cycle in TBS/Tween-20.
7. Develop color using the enhanced DAB reaction. Researchers should empirically determine the suitability of the IGF-1 Clone 1F-3H10 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IGF-1 Clone 1F-3H10 n/a product has the following accession number(s) (GI #1247519) (NCBI Accession #CAA01954.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
This ascites fluid contains mouse monoclonal antibody 1F6-3H10 raised against recombinant hIGF-1. This monoclonal has been found to bind specifically to hIGF-1 by indirect ELISA. western immunoblots. and immunocytochemistry. This monoclonal antibody was tested for recognition of insulin. IGF-2. and other growth factors by ELISA techniques. It has been found to be a mouse IgG1 Kappa by isotyping.