anti-IGF-1 Clone 1F-3H10 antibody product blog
Tags: Antibody; Monoclonal Antibody; IGF-1 Clone 1F-3H10; anti-IGF-1 Clone 1F-3H10 antibody;
The IGF-1 Clone 1F-3H10 n/a (Catalog #MBS350014) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-human Insulin-like Growth Factor-1 Monoclonal Antibody 1F6-3H10 reacts with HumanCrossreactivity Note: No cross reaction with insulin or IGF-2 and may cross-react with other species as described in the data sheet. MyBioSource\'s IGF-1 Clone 1F-3H10 can be used in a range of immunoassay formats including, but not limited to, Indirect ELISA, Western Immunoblot, Western Blot.
ELISA: This IgG fraction has been found by indirect ELISA to bind to hIGF-1 at concentrations of 50 ng/well or less.
Western Blot: Western immunoblots resulted in a single band being detected at ~ 7.5 kDa at a dilution of 1:5000
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the monoclonal antibody after dilution at least 25 ng/ml. (Note: higher dilutions may be needed). Use 1% normal goat serum in TBS/Tween-20 as buffer. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-mouse IgG antiserum diluted 1:2500 (Note: dilution may vary depending upon supplier) in 1% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 4 for 5 minutes per cycle in TBS/Tween-20.
7. Develop color using the enhanced DAB reaction or develop film using ECL or other chemiluminescent techniques. Researchers should empirically determine the suitability of the IGF-1 Clone 1F-3H10 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IGF-1 Clone 1F-3H10 n/a product has the following accession number(s) (GI #1247519) (NCBI Accession #CAA01954.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
This IgG fraction contains mouse monoclonal antibody 1F6-3H10 raised against recombinant hIGF-1. This monoclonal antibody has been found to bind specifically to hIGF-1 by indirect ELISA. cytochemistry and western blots. This monoclonal antibody was tested for recognition of insulin. IGF-2. and other growth factors by ELISA techniques. It has been found to be a mouse IgG1 kappa by isotyping. Each vial contains 50 ?g of highly purified IgG which was lyophilized from a volatile buffer solution.
In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IGF-1 Clone 1F-3H10 are readily searchable from our website. Different antibodies against the same target such as IGF-1 Clone 1F-3H10 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.