anti-IgM antibody product blog
Tags: Antibody; IgM; Monoclonal Antibody; anti-IgM antibody;
The IgM n/a (Catalog #MBS666309) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s IgM can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the IgM n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The IgM n/a product has the following accession number(s) (GI #1020017) (NCBI Accession #AAC41922.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Information: Immunoglobulin expressed by the mouse plasmacytoma TEPC 183 is an IgMk isotype of unknown binding specificity. It is used as a negative control when working with human cells.
Cells Tested: Human PBL, CEM, HPB-MLT, Nalm-6, Jurkat, U-937, Molt-4, Raji, Daudi
Performance: Mouse IgM was tested as a negative control in Flow cytometry at 20ug/ml using Goat anti-Mouse IgG/FITC as a 2 degree reagent. All cells tested failed to stain or display a significant mean shift above a buffer background. Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2 um filtered and vialed under aseptic conditions.
Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IgM are readily searchable from our website. Different antibodies against the same target such as IgM may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.