|The IkB alpha, Cleaved n/a (Catalog #MBS602080) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s IkB alpha, Cleaved can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
Suitable for use in Western Blot.
Dilution: Western Blot: 1ug/ml
Optimal dilution determined by the researcher. Researchers should empirically determine the suitability of the IkB alpha, Cleaved n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). Recent studies have shown that IkBa can be cleaved by caspases during apoptosis to produce an amino-terminal truncated IkBa (DN-IkBa) (7,8). The DN-IkBa is resistant to degradation in response to inducers of NF-kB and act as dominant inhibitory molecule that suppresses NF-kB activity during apoptosis.
Immunogen: Synthetic peptide corresponding to the caspase-3 mediated cleavage site. Positive Control: anti-Fas treated jurkat (30 min). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IkB alpha, Cleaved are readily searchable from our website. Different antibodies against the same target such as IkB alpha, Cleaved may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.