anti-IL-2 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-IL-2 antibody; IL-2;
The IL-2 il-2 (Catalog #MBS666629) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human IL-2-R-PE reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s IL-2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA).1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test). Researchers should empirically determine the suitability of the IL-2 il-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IL-2 il-2 product has the following accession number(s) (GI #4029234) (NCBI Accession #CAA07317.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human IL-2-R-PE with the following immunoassay(s):
Testing Data
Information: The cytokine human interleukin-2 (IL-2) is a 15 kd protein expressed upon activation by peripheral TH1 cells, medullary thymocytes, and a subset of large granular lymphocytes. Secreted IL-2 stimulates proliferation and lymphokine production by T cells, B cells and NK cells.
Performance: Ficoll prepared human peripheral blood mononuclear cells were stimulated 4 hours in the presence of 50 ng/ml PMA, 2 muM ionomicin and 5 muM monensin. Cells were then harvested, washed and fixed for 30 min with 2% buffered formaldehyde after which they were washed two times. Fixed cells were permeabolized 10 minutes in a buffer containing 0.3% Saponin. Subsequent reagent incubations and washes were done using this buffer. Five x 105 cells per tube were pre incubated 5 minutes with 20 mul of 250 mug/ml human IgG (to block nonspecific binding), after which they were incubated 45 minutes on ice with 80 mul of anti-IL-2/R-PE at a 1:50 dilution (5 mug/ml). Cells were washed three times, fixed and analyzed by FACS using a lymphocyte gate. A net 19% sub population of the cells stained positive with a mean shift of 1.0 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 mul of 0.5 mg/ml anti-IL-2 antibody. Conjugation: PE
Production: Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.25 mg/ml with an A565/A280 ratio of 3.35.
Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: Recombinant human IL-2. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IL-2 are readily searchable from our website. Different antibodies against the same target such as IL-2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.