anti-IRF3 antibody product blog
Tags: Antibody; anti-IRF3 antibody; IRF3; Monoclonal Antibody;
The IRF3 irf3 (Catalog #MBS9215402) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IRF3 Antibody reacts with Human, African Green Monkey and may cross-react with other species as described in the data sheet. MyBioSource\'s IRF3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).WB ~~ 1:2000. Researchers should empirically determine the suitability of the IRF3 irf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IRF3 irf3 product has the following accession number(s) (GI #308199446) (NCBI Accession #NP_001184051.1) (Uniprot Accession #Q14653). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the IRF3 Antibody with the following immunoassay(s):
Western Blot (WB) (All lanes : Anti-IRF3 Antibody at 1:2000 dilution
Lane 1: COS-7 whole cell lysate
Lane 2: Daudi whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: HT-29 whole cell lysate
Lane 5: Jurkat whole cell lysate
Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 mug per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 47 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.)
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
Antigen Source: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IRF3 are readily searchable from our website. Different antibodies against the same target such as IRF3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.