anti-IRS1 antibody product blog
Tags: Antibody; Monoclonal Antibody; IRS1; anti-IRS1 antibody;
The IRS1 irs1 (Catalog #MBS609820) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IRS1, phosphorylated (Ser302 mouse/ Ser307 human) (Insulin receptor substrate 1, HIRS-1, Insulin receptor substrate 1, IRS-1) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s IRS1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF).Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry.
Dilution: Western Blot: 1:1000 for HEK293 and transfected CHO cells.
Immunoprecipitation: 1:1000 for 5ul/ml IR/IRS1 transfected CHO cells.
Immunocytochemistry: 1:500 for IR/IRS transfected CHO cells. Researchers should empirically determine the suitability of the IRS1 irs1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IRS1 irs1 product has the following accession number(s) (GI #5031805) (NCBI Accession #NP_005535.1) (Uniprot Accession #P35568). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the IRS1, phosphorylated (Ser302 mouse/ Ser307 human) (Insulin receptor substrate 1, HIRS-1, Insulin receptor substrate 1, IRS-1) with the following immunoassay(s):
Western Blot (WB) (Western Blot: Untreated (lanes 1, 3, 5) and Calyculin A/Okadaic Acid-treated (lanes 2, 4, 6) IR/IRS1 transfected CHO cell lysate was resolved by electrophoresis, transferred to PVDF and probed with MBS609820 (1:1000 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemilumin- escence detection system. Arrow indicates phospho Ser302 IRS1 at ~185kD.)
Western Blot (WB) (Western Blot: Untreated (lanes 1) and Insulin-treated (lanes 2) HEK293 cell lysate was resolved by electrophoresis, transferred to PVDF and probed with MBS609820(1:1000 dilution). Proteins were visualized using a goat anti- mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates IRS1 phosphorylated on Ser302 (~185kD).)
IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. IRS1 is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer. Some of the more notable phosphorylation sites are Ser302 that is phosphorylated following insulin stimulation. Ser307, phosphorylated by JNK and IKK, is a key regulatory site that appears to disrupt the IRS1/IR interaction and inhibits insulin-mediated activation of the PI3 kinase and MAPK pathways, and Ser636/639 that is known to be phosphorylated by p70S6K downstream of mTOR and acts as a negative feedback loop.