anti-KDEL antibody product blog
Tags: Antibody; Monoclonal Antibody; KDEL; anti-KDEL antibody;
The KDEL n/a (Catalog #MBS809073) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KDEL Antibody, Clone 1F2: HRP reacts with Human; Mouse; Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s KDEL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).WB: 1:1000. Researchers should empirically determine the suitability of the KDEL n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the KDEL Antibody, Clone 1F2: HRP with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 1F2. Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL. Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)
The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.
Target: KDEL
Immunogen: KDEL peptide (C-AVKDEL-COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)
Conjugate: HRP
Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing KDEL are readily searchable from our website. Different antibodies against the same target such as KDEL may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.