anti-Kdelr1 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-KDELR1 antibody; KDELR1; KDEL;
The Kdelr1 kdelr1 (Catalog #MBS804623) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KDEL Receptor Antibody: ATTO 390 reacts with Human, Monkey, Rat, Mouse, Hamster, Rabbit, Pig, Bovine, Sheep, Canine, Chicken, Drosophilia, Xenopus and may cross-react with other species as described in the data sheet. MyBioSource\'s KDEL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF).1:1000 dilution was sufficient for detection by colorimetric analysis. Researchers should empirically determine the suitability of the Kdelr1 kdelr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Kdelr1 kdelr1 product has the following accession number(s) (GI #19527170) (NCBI Accession #NP_598711.1) (Uniprot Accession #Q99JH8). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the KDEL Receptor Antibody: ATTO 390 with the following immunoassay(s):
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10. Tissue: NRK cells. Species: Rat. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. KR-10 staining red; DAPI staining blue. Merged images. Courtesy of: Institute of Mol. and Cell Bio, Singapore.)
Western Blot (WB) (Western Blot analysis of Rat tissue lysate showing detection of KDEL Receptor protein using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Background Info: Detects an ~25kDa protein corresponding to the molecular mass of KDEL on SDS-PAGE immunoblots.
Scientific Background: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity; and all are located within the cis-Golgi and its intermediate compartments (4). In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).