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anti-KIF5B antibody product blog

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Posted on 2016-01-12 20:52:38 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; anti-KIF5B antibody; KIF5B; Kinesin Heavy Chain / KIF5B;
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The KIF5B kif5b (Catalog #MBS246591) is an Antibody produced from Kinesin Heavy Chain/ KIF5B antibody was produced in Mouse. and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone KN-03] (IgM) to Pig Kinesin Heavy Chain / KIF5B reacts with Human, Mouse, Pig, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Kinesin Heavy Chain / KIF5B can be used in a range of immunoassay formats including, but not limited to, IHC This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody.
IHC-Paraffin (10 ug/mL)
ICC

(Optimal dilution to be determined by the researcher). Researchers should empirically determine the suitability of the KIF5B kif5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The KIF5B kif5b product has the following accession number(s) (GI #4758648) (NCBI Accession #NP_004512.1) (Uniprot Accession #P33176). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Monoclonal [clone KN-03] (IgM) to Pig Kinesin Heavy Chain / KIF5B with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-KIF5B antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunohistochemistry (IHC) KIF5B.

Immunohistochemistry (IHC) (Anti-KIF5B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunohistochemistry (IHC) KIF5B.

Immunogen Species: Kinesin Heavy Chain/ KIF5B antibody was raised against Pig.
Antigen Type: Protein fraction
Immunogen: kinesin Heavy Chain/KIF5B antibody was raised against enriched fraction of porcine brain kinesin. .
Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply an universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip.
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