anti-KIT antibody product blog
Tags: Antibody; Monoclonal Antibody; KIT; anti-KIT antibody; c-kit Oncoprotein;
The KIT kit (Catalog #MBS570331) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human c-kit Oncoprotein (CD117) (T595) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s c-kit Oncoprotein can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin.The T595 antibody is suitable for the detection of the c-kit protein by immunocytochemistry on isolated cells, and immunohistochemistry on frozen and formalin fixed paraffin embedded tissues. For the latter application the high temperature antigen unmasking technique with 1 mM EDTA (pH 8.0) should be used (two cycles of 10 minutes in a microwave oven at 500 W ; see reference 5), and incubation with the primary antibody for 60 min. at 25 degree C. Optimal antibody dilutions for the different applications should be determined by titration; recommended dilution 1:20 -1:40. Researchers should empirically determine the suitability of the KIT kit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The KIT kit product has the following accession number(s) (GI #4557695) (NCBI Accession #NP_000213.1) (Uniprot Accession #P10721). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
The c-kit proto-oncogene encodes a transmembrane receptor with tyrosine kinase activity, c-kit (CD117), also known as stem cell factor receptor (SCFR), which is closely related to the platelet-derived growth factor receptor family. The c-kit oncoprotein plays a role during normal hematopoiesis, gametogenesis and melanogenesis. It is expressed in among others normal breast epithelium, melanocytes, mast cells and glial cells.
Defects in c-kit are a cause of several diseases, such as gastrointestinal stromal tumor (GIST), and testicular germ cell tumor. Also acute myelogenous leukemia (AML), a malignant disease in which hematopoietic precursors are arrested in an early stage of development, is related to mutation in c-kit. As a result, the antibody to CD117 is suitable the characterization of certain leukemias.
Source Note: T595 is a mouse monoclonal IgG1 antibody derived by fusion of p3-NS1-Ag4-1 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with prokaryotic recombinant protein corresponding to the three N-terminal C2-like extracellular domains of the human c-kit oncoprotein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing KIT are readily searchable from our website. Different antibodies against the same target such as KIT may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.