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anti-KRT17 antibody product blog

Posted on 2016-06-03 15:42:23 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; anti-KRT17 antibody; KRT17; Keratin 17;
The KRT17 krt17 (Catalog #MBS200354) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KRT17 antibody (clone AT9F3) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Keratin 17 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).
Recommended starting dilution is 1:1000. Researchers should empirically determine the suitability of the KRT17 krt17 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The KRT17 krt17 product has the following accession number(s) (GI #4557701) (NCBI Accession #NP_000413). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the KRT17 antibody (clone AT9F3) with the following immunoassay(s):
Western Blot (WB) (The Recombinant Human KRT17 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT17 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant Human KRT17
Lane 2.: HeLa cell lysate
Lane 3.: A431 cell lysate)
Western Blot (WB) KRT17.

Anti-human KRT17 mAb, clone AT9F3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human KRT17 protein.
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments. Keratin 17 is involved in wound healing and cell growth, two processes that require rapid cytoskeletal remodeling. Keratinocytes deficient in keratin 17 exhibit abnormal Akt/mTOR signaling and fail to produce an increase in translation, cell size, or growth; these cells also exhibit abnormal 14-3-3 sigma localization.

Immunogen: Recombinant human KRT17(1-432aa) purified from E Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing KRT17 are readily searchable from our website. Different antibodies against the same target such as KRT17 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.
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