anti-KRT18 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-KRT18 antibody; KRT18; KRT18 / CK18 / Cytokeratin 18;
The KRT18 krt18 (Catalog #MBS245643) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone AT2G3] (IgG2b,k) to Human KRT18 / CK18 / Cytokeratin 18 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s KRT18 / CK18 / Cytokeratin 18 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA).ELISA, IF (1:500), IHC-P (10 ug/ml), WB (1:1000). Researchers should empirically determine the suitability of the KRT18 krt18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The KRT18 krt18 product has the following accession number(s) (GI #4557888) (NCBI Accession #NP_000215.1) (Uniprot Accession #P05783). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Monoclonal [clone AT2G3] (IgG2b,k) to Human KRT18 / CK18 / Cytokeratin 18 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-KRT18 / Cytokeratin 18 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunofluorescence (IF) (Immunofluorescence of HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human KRT18 antibody (1:500) with Texas Red (Red).)
Western Blot (WB) (Cell lysates of HeLa and AGS (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT18 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis.
Target Species: Human
Immunogen Description: Recombinant human KRT18 (79-430aa) purified from E Coli. Immunogen Type: Recombinant protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing KRT18 are readily searchable from our website. Different antibodies against the same target such as KRT18 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.