anti-KRT23 antibody product blog
Tags: Antibody; Monoclonal Antibody; KRT23; anti-KRT23 antibody;
The KRT23 krt23 (Catalog #MBS200254) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KRT23 antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s KRT23 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF).WB: 1:500 ~ 1:3000. Researchers should empirically determine the suitability of the KRT23 krt23 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The KRT23 krt23 product has the following accession number(s) (GI #27894339) (NCBI Accession #NP_056330) (Uniprot Accession #Q9C075). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the KRT23 antibody with the following immunoassay(s):
Western Blot (WB) (The cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT23 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Immunofluorescence (IF) (ICC/IF analysis of KRT23 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT23 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
KRT23, also known as cytokeratin 23, is a 422 amino acid intermediate filament protein. The human KRT23 gene is located on chromosome 17q21.2. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, and maintenance of epithelial cells structure. Cytokeratins are described to be differentiation markers in several epithelial cancers.
Antigen Species: Human
Immunogen: Recombinant human KRT23 (271-422aa) purified from E. coli. Clone Note: Anti-human KRT23 mAb, clone AT2F6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human KRT23 protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing KRT23 are readily searchable from our website. Different antibodies against the same target such as KRT23 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.