|The Kv3.2 kcnc2 (Catalog #MBS806992) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Rat Kv3.2 Monoclonal IgG1 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Kv3.2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF).
Western Blot: 1:1000. Researchers should empirically determine the suitability of the Kv3.2 kcnc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Kv3.2 kcnc2 product has the following accession number(s) (GI #41281706) (NCBI Accession #NP_631962.1) (Uniprot Accession #P22462). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Anti-Rat Kv3.2 Monoclonal IgG1 with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Mouse, Rat Brain showing detection of ~80-100 kDa Kv3.2 protein using Mouse Anti-Kv3.2 Monoclonal Antibody, Clone S410-17. Lane 1: MW Ladder. Lane 2: Mouse Brain. Lane 3: Rat Brain Membrane. Lane 4: Rat Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Kv3.2 Monoclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~80-100 kDa. Other Band(s): ~30 kDa for Rat Brain.)
Kv3.2, a member of the KCNC2 gene family, belongs to a group of potassium channels that contribute to the maintenance of cell volume, membrane potential, neuronal excitability and the secretion of transmitters, salt and hormones. Kv3.2 are predominantly expressed in neurons that fire at high frequencies, and in the fast spiking GABAergic interneurons of the neocortex, hippocampus, and caudate nucleus (1-3). They specifically functioning as a delayed rectifier activated by large membrane depolarizations (2, 4).
Immunogen: Fusion protein amino acids 474-613 (Cytoplasmic C-terminus) of rat Kv3.2a
Immunogen Species: Rat. COA: A 1:100 dilution was sufficient for detection of Kv3.2 in 20 ug of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Storage Buffer: PBS pH7.4, 50% glycerol, 0.1% sodium azide
Conjugate: ATTO 633
Cellular Localization: Cell Membrane. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Kv3.2 are readily searchable from our website. Different antibodies against the same target such as Kv3.2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.