anti-LAMA3 antibody product blog
Tags: Antibody; Monoclonal Antibody; LAMA3; anti-LAMA3 antibody; LAMA3/Laminin 5;
The LAMA3 lama3 (Catalog #MBS1750009) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-LAMA3/Laminin 5 Rabbit Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s LAMA3/Laminin 5 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB).WB: 1:500-1:2000
ICC/IF: 1:50-1:200. Researchers should empirically determine the suitability of the LAMA3 lama3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The LAMA3 lama3 product has the following accession number(s) (GI #732170493) (NCBI Accession #NP_000218.3) (Uniprot Accession #Q16787). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-LAMA3/Laminin 5 Rabbit Monoclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of LAMA3 expression in HeLa cell lysate (MBS1750009).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMA3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LAMA3 )
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Immunogen: A synthesized peptide derived from human LAMA3
Subcellular Localization: Secreted, extracellular space, extracellular matrix, basement membrane. Major component.
Tissue Specificity: Skin; respiratory, urinary, and digestive epithelia and in other specialized tissues with prominent secretory or protective functions. Epithelial basement membrane, and epithelial cell tongue that migrates into a wound bed. A differential and focal expression of the subunit alpha-3 is observed in the CNS. Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing LAMA3 are readily searchable from our website. Different antibodies against the same target such as LAMA3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.