anti-LAMP3 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-LAMP3 antibody; LAMP3;
The LAMP3 lamp3 (Catalog #MBS6005565) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The LAMP3 (Lysosome-associated Membrane Glycoprotein 3, LAMP-3, Lysosomal-associated Membrane Protein 3, DC-lysosome-associated Membrane Glycoprotein, DC LAMP, Protein TSC403, CD208, DCLAMP, TSC403) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s LAMP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1ug/ml
Immunohistochemistry: 1:200. Researchers should empirically determine the suitability of the LAMP3 lamp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The LAMP3 lamp3 product has the following accession number(s) (GI #38455385) (NCBI Accession #NP_055213.2) (Uniprot Accession #Q9UQV4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the LAMP3 (Lysosome-associated Membrane Glycoprotein 3, LAMP-3, Lysosomal-associated Membrane Protein 3, DC-lysosome-associated Membrane Glycoprotein, DC LAMP, Protein TSC403, CD208, DCLAMP, TSC403) with the following immunoassay(s):
Testing Data (MCF-7 cell lysate was probed with MBS6005565 (1ug/ml). Proteins were visualized using an IgG (HRP) goat anti-mouse IgG secondary antibody and visualized using a chemiluminescence detection system.|Arrow indicates LAMP3 (~65kD).)
Immunohistochemistry (IHC) (Immunohistochemistry analysis: Representative Lot Data. Paraffin-embedded colorectal adenocarcinoma (Fig. 1 & 2) tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using MBS6005565 (1:200). Positive staining was observed in colorectal adenocarcinoma tissue.)
Lysosyme-associated membrane proteins (LAMP) are transmembrane lysosomal glycoproteins, which share a common Gly-Tyr motif as a lysosomal targeting signal. While LAMP-1 and LAMP-2 contain type 1 transmembrane domains, LAMP-3 contains a transmembrane 4 superfamily domain, tetraspanin. FceRI-mediated stimulation of basophils causes LAMP-3 to be translocated from its intracellular localization to the plasma membrane. LAMP-3 thereby serves as a reliable marker for basophil activation. LAMP-3 expression is known to be reported in human bone marrow mast cells and in leukemic human mast cell line (HMC-1), among other cell lines. LAMP3 is shown to be regulated by hypoxia in a panel of tumor cells, via the unfolded protein response (UPR). UPR is a mechanism of adaptation to endoplasmic reticulum stress and is demonstrated to contribute to hypoxic adaptation in tumors through multiple mechanisms.
Immunogen: Recombinant corresponding to human LAMP-3. Positive Control: MCF-7 cell lysate. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing LAMP3 are readily searchable from our website. Different antibodies against the same target such as LAMP3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.