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anti-LGALS3 antibody product blog

Posted on 2017-06-08 06:51:29 by mybiosource_staff
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Tags: Antibody; anti-LGALS3 antibody; LGALS3; Monoclonal Antibody;
The LGALS3 lgals3 (Catalog #MBS9131092) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The LGALS3 Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s LGALS3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the LGALS3 lgals3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The LGALS3 lgals3 product has the following accession number(s) (GI #215274262) (NCBI Accession #P17931.5) (Uniprot Accession #P17931). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the LGALS3 Monoclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using LGALS3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (MBS128212) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.)
Western Blot (WB) LGALS3.

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.

Immunogen: Recombinant protein of human LGALS3. Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing LGALS3 are readily searchable from our website. Different antibodies against the same target such as LGALS3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. LGALS3 also interacts with the following gene(s): DDOST, EGFR, GEMIN4, HRAS, KRAS, LGALS3BP, MUC1, NRAS, PRKCSH, SUFU.
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