anti-LGR4 antibody product blog
Tags: Antibody; Monoclonal Antibody; LGR4; anti-LGR4 antibody;
The LGR4 lgr4 (Catalog #MBS468010) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-LGR4 (GPR48) monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s LGR4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), CELISA.Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400. Researchers should empirically determine the suitability of the LGR4 lgr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The LGR4 lgr4 product has the following accession number(s) (GI #157694513) (NCBI Accession #NP_060960.2) (Uniprot Accession #Q9BXB1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-LGR4 (GPR48) monoclonal antibody with the following immunoassay(s):
Testing Data (Fig.1: FACS analysis of BOSC23 cells using BBX-1H1 Cat.# GM- 0606. BOSC23 cells were transiently transfected with an expression vector encoding either LGR4-hum (red curve), LGR4-murine (blue curve) or an irrelevant protein (control transfectant: black curve). Binding of BBX-3A8 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with LGR4-hum transfected cells.)
Testing Data #2 (SDS page analysis of BBX-1H1 The antibody was purified by protein G affinity chromatography from cell culture supernatants Fig.2: SDS-PAGE analysis of purified BBX-1H1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified BBX-1H1 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Background: LGR4 (Leucine-rich repeat- containing G protein-coupled receptor 4) belongs to the superfamily of G protein-coupled receptors (GPCRs). It is a 951 amino acid multi-pass membrane protein and has multiple N-terminal leucine-rich repeats, which are important for interaction with the glycoprotein ligands, and 7 transmembrane domains (1,3). LGR4 is highly expressed in the adult human pancreas but also with moderate levels of expression in placenta, kidney, brain and heart (2). LGR4 functions as an orphan receptor that may be involved in physiologic activities throughout the cell. It is overexpressed in various cancer types and is thought to enhance carcinoma invasiveness and metastasis, suggesting an important role in tumor progression.
Buffer: phosphate buffered saline, pH 7.2
Immunogen: genetic immunisation with cDNA encoding human LGR4
Selection: based on recognition of the complete native protein expressed on transfected mammalian cells
Immunogen: cDNA. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing LGR4 are readily searchable from our website. Different antibodies against the same target such as LGR4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.