anti-LIN28 antibody product blog
Tags: Antibody; Monoclonal Antibody; LIN28; anti-LIN28 antibody;
The LIN28 lin28a (Catalog #MBS857854) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The LIN28 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s LIN28 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the LIN28 lin28a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The LIN28 lin28a product has the following accession number(s) (GI #13375938) (NCBI Accession #NP_078950.1) (Uniprot Accession #Q9H9Z2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the LIN28 Antibody with the following immunoassay(s):
Testing Data (Western blot detection of LIN28 in NTERA & F9 cell lysates using LIN28 antibody(1:1000 diluted). Predicted band size:23KDa,Observed band size:23KDa.)
Acts as a �translational enhancer�, driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5�-GGAG-3� motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5�-GGAG-3� motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
Immunogen: Purified recombinant human LIN28 protein fragments expressed in E Coli
Cellular Localization: Cytoplasm; Nucleus. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing LIN28 are readily searchable from our website. Different antibodies against the same target such as LIN28 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.