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anti-LIPA antibody product blog

Posted on 2018-06-23 02:30:43 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; anti-LIPA antibody; LIPA; LIPA/Lysosomal Acid Lipase;
The LIPA lipa (Catalog #MBS2401342) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-LIPA/Lysosomal Acid Lipase Antibody (clone 1F9) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s LIPA/Lysosomal Acid Lipase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB).
IHC-P: 10 ug/ml. Researchers should empirically determine the suitability of the LIPA lipa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The LIPA lipa product has the following accession number(s) (GI #51317399) (NCBI Accession #NP_000226.2) (Uniprot Accession #P38571). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-LIPA/Lysosomal Acid Lipase Antibody (clone 1F9) with the following immunoassay(s):
Immunohistochemistry (IHC) (Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC) LIPA.

Western Blot (WB) (Western Blot analysis of LIPA expression in transfected 293T cell line by LIPA monoclonal antibody (M01), clone 1F9.Lane 1: LIPA transfected lysate(45 KDa).Lane 2: Non-transfected lysate.)
Western Blot (WB) LIPA.

Immunoprecipitation (IP) (Immunoprecipitation of LIPA transfected lysate using anti-LIPA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LIPA rabbit polyclonal antibody.)
Immunoprecipitation (IP) LIPA.

ELISA (EIA) (Detection limit for recombinant GST tagged LIPA is approximately 10 ng/ml as a capture antibody.)

intracellular hydrolysis of cholesteryl esters and triglycerides thathave been internalized via receptor-mediated endocytosis of lipoproteinparticles. Important in mediating the effect of LDL (low densitylipoprotein) uptake on suppression of hydroxymethylglutaryl-CoAreductase and activation of endogenous cellular cholesteryl esterformation.

Immunogen: LIPA (AAH12287, aa1-399 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugation: Unconjugated. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing LIPA are readily searchable from our website. Different antibodies against the same target such as LIPA may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. LIPA also interacts with the following gene(s): CH25H, CYP7A1, SOAT1. The following patways have been known to be associated with this gene. Breast Neoplasms, Carcinoma, Hepatocellular, Cardiovascular Diseases, Cholesterol Ester Storage Disease, Fibrosis, Inflammation, Liver Cirrhosis, Liver Diseases, Lysosomal acid lipase deficiency, Nervous System Diseases are some of the diseases may be linked to Anti-LIPA/Lysosomal Acid Lipase Antibody (clone 1F9).
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