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anti-LRRK2 antibody product blog

Posted on 2016-01-17 08:43:47 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; LRRK2; anti-LRRK2 antibody; LRRK2/Dardarin;
The LRRK2 lrrk2 (Catalog #MBS804337) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The LRRK2/Dardarin Antibody: PE/ATTO 594 reacts with Human, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s LRRK2/Dardarin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC).
1:100 (WB). Researchers should empirically determine the suitability of the LRRK2 lrrk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The LRRK2 lrrk2 product has the following accession number(s) (GI #171846278) (NCBI Accession #NP_940980) (Uniprot Accession #Q5S007). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the LRRK2/Dardarin Antibody: PE/ATTO 594 with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Rat Brain Membrane showing detection of >200 kDa LRRK2/Dardarin protein using Mouse Anti-LRRK2/Dardarin Monoclonal Antibody, Clone S231B-34. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: >200 kDa.)
Western Blot (WB) LRRK2.

Background Info: Detects > 200 kDa protein corresponding to the molecular weight of LRRK2

Scientific Background: LRRK2 is a large protein with multiple domains including several ankyrin, leucine-rich, and WD40 repeats, a Ras-like small GTPase family domain named Roc, and a kinase domain that is closely related to the RIP kinase domain. LRRK2 gene is expressed in brain as well as in other tissues such as lung, liver and heart. LRRK2 might be central to the pathogenesis of several major neurodegenerative diseases associated with parkinsonism. Several dominantly inherited missense mutations in the gene encoding LRRK2 have been identified in several families that exhibit a broad spectrum of neuropathological features.

Conjugate: ATTO 594. Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing LRRK2 are readily searchable from our website. Different antibodies against the same target such as LRRK2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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