anti-Ltb antibody product blog
Tags: Antibody; Monoclonal Antibody; LTB; anti-LTb antibody; LTbeta receptor;
The Ltb ltb (Catalog #MBS584069) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The LTbeta receptor, Mouse, mAb 5G11 reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s LTbeta receptor can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FS, Immunoassay (IA), Western Blot (WB).Application Use: For flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For agonistic studies in vitro, dilutions have to be made according to the amounts of ligand to be activated. Researchers should empirically determine the suitability of the Ltb ltb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Ltb ltb product has the following accession number(s) (GI #6678730) (NCBI Accession #NP_032544.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Product Description: The monoclonal antibody 5G11 reacts with the mouse lymphotoxin beta-receptor (LTbetaR), a member of the tumor necrosis factor (TNF) family. This receptor can be activated by its functional ligands lymphotoxin-alpha1-beta2 (LT-alpha1beta2) and LIGHT and is critically involved in controlling bacterial infections. In Mycobacterium infection, its role is related to bactericidal granuloma formation in the spleen, macrophage differentiation and the maintenance of the appropriate Th1/Th2 cytokine balance. In CMV infection, LTbetaR signaling induces the expression of IFN-beta in infected fibroblasts, resulting in viral stasis. LTbetaR plays an important role in lymphoid organogenesis and tumor development. The receptor is expressed on fibroblasts and stromal cells and at low levels in some myeloid cell lines. In the adult animal, the LTbetaR seems to be necessary for maintaining the splenic architecture and aspects of Ig formation and B cell follicular structure. When the LT-alpha1beta2/LTbetaR pathway is disrupted by genetic deletion, mice completely lack Peyer\'s patches. Also, LTbetaR induces cell death in some adenocarcinoma tumor lines in the presence of IFNgamma. The monoclonal antibody 5G11 directed against the mouse LTbetaR is able to activate the LTbetaR in an agonistic way and induces NFkappaB activation and secretion of MIP-2 and IL-6 in mouse fibroblast cells. Therefore, the monoclonal antibody 5G11 can be used to gain more insight into the expression pattern of the LTbetaR and also to investigate molecular mechanisms induced by LTbetaR activation.
In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Ltb are readily searchable from our website. Different antibodies against the same target such as Ltb may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.