anti-Lys-Asp-Glu-Leu; Lysine-aspartic acid-glutamate-leucine antibody product blog
Tags: Antibody; Monoclonal Antibody; KDEL; anti-Lys-Asp-Glu-Leu; Lysine-aspartic acid-glutamate-leucine antibody; Lys-Asp-Glu-Leu; Lysine-aspartic acid-glutamate-leucine;
The KDEL hspa5 (Catalog #MBS565113) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KDEL monoclonal antibody (10C3) reacts with Avian, Insect, Mammalian, Plant, Yeast and may cross-react with other species as described in the data sheet. MyBioSource\'s KDEL can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemsitry (IHC)-Paraffin, Immunoprecipitation (IP), Western Blot (WB), Electron Microscopy (EM).Western Blot (1:1,000, colorimetric). Detects three bands of ~94kDa, ~78 kDa and ~55kDa by Western blot.
Optimal conditions muse be determined by individually for each application. Researchers should empirically determine the suitability of the KDEL hspa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The KDEL hspa5 product has the following accession number(s) (GI #121574) (NCBI Accession #P06761.1) (Uniprot Accession #P06761). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the KDEL monoclonal antibody (10C3) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis: Lane 1: Grp78 (BiP) recombinant protein, Lane 2: RK-13, Lane 3: Mouse liver microsomes, Lane 4: Rat liver microsomes, Lane 5: HeLa Cell Lysate (heat shocked).)
Immunofluorescence (IF) (Immunofluorescence analysis of endoplasmic reticulum staining of mouse C2C12 myoblasts transfected with wild type mouse ADAM12 using KDEL (Grp78, Grp94) mAb (10C3).)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of human spleen tissue stained with KDEL, mAb (10C3) at 10µg/ml.)
The tetrapeptide KDEL, located at the carboxy-terminal sequences of luminal proteins, is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. KDEL proteins perform essential functions in the endoplasmic reticulum (ER) related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the ER is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor (Erd2p), which is a membrane protein localized in the Golgi apparatus.
Immunogen: Synthetic peptide corresponding to aa 649-654 (S649EKDEL654) of rat Grp78. Source: Purified from ascites. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing KDEL are readily searchable from our website. Different antibodies against the same target such as KDEL may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.