anti-MACROPHAGES/MONOCYTES antibody product blog
Tags: Antibody; Monoclonal Antibody; MACROPHAGES/MONOCYTES; anti-MACROPHAGES/MONOCYTES antibody;
The MACROPHAGES/MONOCYTES n/a (Catalog #MBS219895) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s MACROPHAGES/MONOCYTES can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS)*.Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/5; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose. Researchers should empirically determine the suitability of the MACROPHAGES/MONOCYTES n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HUMAN MACROPHAGES:APC with the following immunoassay(s):
Testing Data (Staining of human peripheral blood granulocytes with Mouse anti Human macrophages:FITC (MBS211113). Permeabilised with Leucoperm)
Testing Data (Staining of human peripheral blood granulocytes with Mouse anti Human macrophages (MBS219286). Permeabilised with Leucoperm)
Testing Data (Macrophages stained with Mouse anti Human Macrophages/ Monocytes (MBS216638) using enhanced DAB in experimental allergic marmoset brain. Mag. X 400)
Testing Data (Staining of human peripheral blood granulocytes with Mouse anti Human Macrophages:RPE (MBS224589). Permeabilised with Leucoperm)
Mouse anti Human macrophages, clone MAC387 recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen recognized by clone MCA387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.
Reconstitution: Reconstitute with 1.0 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Buffer Solution: Phosphate buffered saline
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MACROPHAGES/MONOCYTES are readily searchable from our website. Different antibodies against the same target such as MACROPHAGES/MONOCYTES may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.