anti-MAP2K4 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-Map2k4 antibody; Map2k4;
The MAP2K4 map2k4 (Catalog #MBS850516) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MAP2K4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s MAP2K4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA).WB: 1:500-1:2000
IHC: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000. Researchers should empirically determine the suitability of the MAP2K4 map2k4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MAP2K4 map2k4 product has the following accession number(s) (GI #4506889) (NCBI Accession #NP_003001.1) (Uniprot Accession #P45985). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MAP2K4 Antibody with the following immunoassay(s):
Western Blot (WB) (Figure 1: Western blot analysis using MAP2K4 mouse mAb against HepG2 (1), K562 (2), and HEK293 (3) cell lysate.)
Immunohistochemistry (IHC) (Figure 2: Immunohistochemical analysis of paraffin-embedded muscle tissues using MAP2K4 mouse mAb with DAB staining.)
Testing Data
This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.Tissue specificity: Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Immunogen: Purified recombinant fragment of human MAP2K4 expressed in E. Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MAP2K4 are readily searchable from our website. Different antibodies against the same target such as MAP2K4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Bone, Brain, Kidney, Liver, Lung, Muscle, Nerve, Prostate, Skin, Vascular tissues are correlated with this protein.