anti-MBNL1 antibody product blog
Tags: Antibody; Monoclonal Antibody; MBNL1; anti-MBNL1 antibody;
The MBNL1 mbnl1 (Catalog #MBS6009904) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MBNL1 (Muscleblind-like Protein 1, Triplet-expansion RNA-binding Protein, EXP, KIAA0428, MBNL) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s MBNL1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Dilution: Immunofluorescence: 10ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml. Researchers should empirically determine the suitability of the MBNL1 mbnl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MBNL1 mbnl1 product has the following accession number(s) (GI #46411174) (NCBI Accession #NP_997180.1) (Uniprot Accession #Q9NR56). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MBNL1 (Muscleblind-like Protein 1, Triplet-expansion RNA-binding Protein, EXP, KIAA0428, MBNL) with the following immunoassay(s):
Western Blot (WB) (MBNL1 monoclonal antibody Western Blot analysis of MBNL1 expression in Hela NE.)
Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to MBNL1 on HeLa cell (antibody concentration 10ug/ml).)
Testing Data (Detection limit for recombinant GST tagged MBNL1 is approximately 3ng/ml as a capture antibody.)
Immunohistochemistry (IHC) (Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC) (Human Skeletal muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))
Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5\'-YGCU(U/G)Y-3\'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.
Immunogen: Recombinant corresponding to aa1-383 from human MBNL1 with GST tag. MW of the GST tag alone is 26kD. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MBNL1 are readily searchable from our website. Different antibodies against the same target such as MBNL1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.