anti-Mfn2 antibody product blog
Tags: Antibody; Monoclonal Antibody; Mitofusin 2; anti-MFN2 antibody; MFN2;
The Mfn2 mfn2 (Catalog #MBS802208) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mitofusin 2 Antibody: RPE reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Mitofusin 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC).1:1000 WB. Researchers should empirically determine the suitability of the Mfn2 mfn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Mfn2 mfn2 product has the following accession number(s) (GI #120407048) (NCBI Accession #NP_573464.2) (Uniprot Accession #Q80U63). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mitofusin 2 Antibody: RPE with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Rat Brain Membrane showing detection of ~90 kDa Mitofusin 2 protein using Mouse Anti-Mitofusin 2 Monoclonal Antibody, Clone S153-5. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Mitofusin 2 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~90 kDa.)
Background Info: Detects a 90 kDa protein. No cross-reactivity against Mitofusin-1.
Scientific Background: Mitofusin-2 (Mfn2) is a membrane protein of the mitochondria and is important in mitochondrial fusion in mammalian cells. Mfn2 activity has also been implicated in mitochondrial metabolism and the loss of Mfn2 may be the reason for certain metabolic modifications that are seen in individuals with obesity. Mfn2 has been associated with maintaining the membrane potential of the mitochondria. Mfn2 has been shown to hinder mitochondrial antiviral signaling by inhibiting production of type 1 interferons. Mfn2 is a p53-inducible target and involved in cell proliferation, apoptosis promotion and tumor suppression.
Conjugate: RPE. Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Mfn2 are readily searchable from our website. Different antibodies against the same target such as Mfn2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.