anti-MHC Class 1, H2, Db, alpha3 antibody product blog
Tags: Antibody; Monoclonal Antibody; MHC Class 1, H2, Db, alpha3; anti-MHC Class 1, H2, Db, alpha3 antibody;
The MHC Class 1, H2, Db, alpha3 n/a (Catalog #MBS602196) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s MHC Class 1, H2, Db, alpha3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry - Quality testedImmunohistochemistry - Reported in literature
Immunoprecipitation - Reported in literature
Blocking - Reported in literature
Complement Mediated Cell Depletion - Reported in literature.
Flow Cytometry
Purified (UNLB) antibody - <= 1 ug/106 cells
FITC, BIOT, and AF488 conjugates - <=1 ug/106 cells
PE conjugate - <= 0.5 ug/106 cells
APC conjugate - <= 0.1 ug/106 cells
For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL.
Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need. Researchers should empirically determine the suitability of the MHC Class 1, H2, Db, alpha3 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MHC Class 1, H2, Db, alpha3 with the following immunoassay(s):
Testing Data
Testing Data (BALB/c mouse splenocytes were stained with Mouse Anti-Mouse H-2Db-UNLB (MBS602196) followed by Goat Anti-Mouse IgG2a, Human ads-FITC (MBS674471).)
The �classical� MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny. The monoclonal antibody 28-14-8 binds to the alpha 3 domain of H-2Db in the presence or absence of beta 2 microglobulin. It cross reacts with the alpha 3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells.
Immunogen: C3H.SW mouse splenocytes. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MHC Class 1, H2, Db, alpha3 are readily searchable from our website. Different antibodies against the same target such as MHC Class 1, H2, Db, alpha3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.