anti-MHC Class I antibody product blog
Tags: Antibody; Monoclonal Antibody; MHC Class I; anti-MHC Class I antibody;
The MHC Class I n/a (Catalog #MBS666614) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human MHC Class I-Biotin reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s MHC Class I can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA). Researchers should empirically determine the suitability of the MHC Class I n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The MHC Class I n/a product has the following accession number(s) (GI #1545844) (NCBI Accession #AAB08079.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human MHC Class I-Biotin with the following immunoassay(s):
Testing Data
Information: Human CD326 (EpCAM, EGP2, HEA) is a 40kD glycoprotein expressed on epithelial cells and many carcinomas. It is an adhesion molecule whose function is implicated in carcinogenesis and erythropoesis. Antibody ANC8D4 recognizes recombinant CD326 in EIA, and cell surface human CD326 in FACS.
Performance: Cultured human SW480 cells were harvested by EDTA. Five x 105 cells per tube were washed and pre incubated ~5 minutes with 20 ul human IgG at 250 ug/ml (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-human CD326/Biotin at concentration of 5 ug/ml. They were then washed twice and incubated with 2 degree reagent Streptavidin/R-PE after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.93 log10 fluorescent units when compared to an Isotype control Mouse IgG1/Biotin. Binding was blocked when cells were pre incubated with unlabeled anti-human CD326 mAb. Conjugation: Biotin
Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: Recombinant Human CD326. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MHC Class I are readily searchable from our website. Different antibodies against the same target such as MHC Class I may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.