anti-MHC CLASS II RT1B antibody product blog
Tags: Antibody; Monoclonal Antibody; MHC CLASS II RT1B; anti-MHC CLASS II RT1B antibody;
The MHC CLASS II RT1B n/a (Catalog #MBS213835) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s MHC CLASS II RT1B can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10. Researchers should empirically determine the suitability of the MHC CLASS II RT1B n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI RAT MHC CLASS II RT1B:RPE with the following immunoassay(s):
Testing Data (Staining of rat spleen cells with Mouse anti Rat RT1B: Alexa Fluor 488 )
Testing Data (Staining of rat spleen cells with Mouse anti Rat RTIB: Alexa Fluor 647 )
Testing Data (Rat Spleen Tissue lysate probed with Mouse anti Rat MHC Class II RT1B)
Testing Data (Staining of rat spleen cells with Mouse anti Rat RT1B: FITC (MBS211026))
Testing Data (Staining of rat spleen cells with Mouse anti Rat RT1B: RPE)
Testing Data (Staining of rat spleen cells with Mouse anti Rat RT1B: FITC (MBS211025))
Testing Data (Staining of rat spleen cells with Mouse anti Rat RT1B (MBS213028))
Testing Data (Staining of rat splenocytes with Mouse anti Rat MHC class II RT1B (MBS213030))
Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 recognizes a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Rat MHC Class II RT1B antibody, clone OX-6 does not react with the rat BDIX strain due to a defect in RT1B expression (Male et al. 1987). The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region. Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region (McMaster and Williams 1979 and Male et al. 1987). This product is routinely tested in flow cytometry on rat splenocytes.
Reconstitution: Reconstitute with 1 ml distilled water
Perservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
Preparation: Purified IgG prepared by affinity chromatography on Protein G. Immunogen: Rat Thymocyte membrane glycoprotein
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Rat.