|The Mitf mitf (Catalog #MBS600026) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Microphthalmia (Transcription Factor, MiTF) (BSA & Azide Free) reacts with Canine, Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Microphthalmia can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Gel Shift Assay (GS/EMSA).
Dilution: Gel Supershift (Order Ab at 1mg/ml)
Western Blotting (Not verified)
Immunohistochemistry (Formalin/paraffin: 4-8ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator. Researchers should empirically determine the suitability of the Mitf mitf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Mitf mitf product has the following accession number(s) (GI #3319231) (NCBI Accession #AAC26170.1) (Uniprot Accession #O88368). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells.There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kD and 56kD, while the longer Mi form runs as a cluster of bands at 60-70kD in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.1.
Immunogen: N-terminal fragment of human Mi protein
Cellular Localization: Nuclear. Positive Control: 501 Mel cells1. Melanoma. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Mitf are readily searchable from our website. Different antibodies against the same target such as Mitf may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Mitf also interacts with the following gene(s): Creb1, Edn3, Kit, Mapk1, Mapk3, Mlana, Sox10, Sox9, Tp53.