anti-MKI67 antibody product blog
Tags: Antibody; Monoclonal Antibody; MKI67; anti-MKI67 antibody;
The MKI67 mki67 (Catalog #MBS9131268) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MKI67 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s MKI67 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the MKI67 mki67 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MKI67 mki67 product has the following accession number(s) (GI #118572663) (NCBI Accession #P46013.2) (Uniprot Accession #P46013). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MKI67 Monoclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of HepG2 cells, using MKI67 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse spleen using MKI67 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human tonsil using MKI67 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human spleen using MKI67 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse testis using MKI67 antibody at dilution of 1:100 (40x lens).)
Immunofluorescence (IF) (Immunofluorescence analysis of A431 cells using MKI67 antibody.)
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.
Immunogen: Recombinant protein of human MKI67. Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MKI67 are readily searchable from our website. Different antibodies against the same target such as MKI67 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. MKI67 also interacts with the following gene(s): BIRC5, CCNA2, CDK1, KIF11, KIF15, MKI67IP, NCAPG, PRC1, TOP2A. Adenocarcinoma, Adenoma, Breast Neoplasms, Carcinoma, Squamous Cell, Colorectal Neoplasms, Hyperplasia, Lung Neoplasms, Necrosis, Neoplasms, Experimental, Nervous System Diseases are some of the diseases may be linked to MKI67 Monoclonal Antibody.