anti-MLANA antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-MLANA antibody; MLANA; MLANA/Mart 1;
The MLANA mlana (Catalog #MBS179788) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MLANA/Mart 1 Rabbit Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s MLANA/Mart 1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB).WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:200. Researchers should empirically determine the suitability of the MLANA mlana for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MLANA mlana product has the following accession number(s) (GI #5031913) (NCBI Accession #NP_005502.1) (Uniprot Accession #Q16655). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-MLANA/Mart 1 Rabbit Monoclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of MLANA expression in Human melanoma tissue lysate (MBS179788).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLANA monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MLANA )
Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
Immunogen: A synthesized peptide derived from human MLANA
Subcellular Localization: Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post- Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.
Tissue Specificity: Expression is restricted to melanoma and melanocyte cell lines and retina.