anti-MMP-3 antibody product blog
Tags: Antibody; MMP-3; anti-MMP-3 antibody; Monoclonal Antibody;
The MMP-3 mmp3 (Catalog #MBS219342) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s MMP-3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB).Western Blot: This item detects a band of approximately 50kDa in cell lysates.
Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Researchers should empirically determine the suitability of the MMP-3 mmp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MMP-3 mmp3 product has the following accession number(s) (GI #4505217) (NCBI Accession #NP_002413.1) (Uniprot Accession #P08254). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HUMAN MMP-3 with the following immunoassay(s):
Testing Data (Staining of paraffin embedded human colon carcinoma with Mouse anti Human MMP-3 following citrate antigen retrieval)
Mouse anti Human MMP-3 antibody, clone 1B4 recognizes human matrix metalloproteinase 3, also known as MMP-3, stromelysin-1 or transin-1. MMP-3 in its pro-form is a 477 amino acid ~54kDa zinc dependent endopeptidase processed to a 378 amino acid, ~45kDa peptide in the mature form containing 4 hemopexin-like repeats. MMPs are also involved in cell proliferation, migration, differentiation and apoptosis (Jana et al. 2012). Most MMP\'s are synthesised as inactive zymogens and a propeptide region must be cleaved off before the enzyme becomes active. Their expression is increased dramatically in a variety of cancer types, where it indicates invasive disease and a poor prognosis (Holmberg et al. 2013). MMP-3 can degrade proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen (Wilhelm et al. 1993). It can activate other MMP\'s and may be involved in the repair processes of the heart after myocardial infarction (Wang et al. 2011). Mouse anti human MMP-3 antibody, clone 1B4 has been used successfully for the identification of MMP-3 in gastric cancer by both Western blotting and immunohistochemistry (Murray et al. 1998).
Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
Preparation: Purified IgG prepared by affinity chromatography on Protein G. Histology Positive Control Tissue: Gastric carinoma
Buffer Solution: Phosphate buffered saline
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MMP-3 are readily searchable from our website. Different antibodies against the same target such as MMP-3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.