|The MMP-8 mmp8 (Catalog #MBS690763) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human MMP-8 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s MMP-8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) - Paraffin.
1. Western blot: Yes (1:400-1000). 2. Immunohistochemistry (Paraffin): Yes (1:50-200). Researchers should empirically determine the suitability of the MMP-8 mmp8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MMP-8 mmp8 product has the following accession number(s) (GI #4505221) (NCBI Accession #NP_002415.1) (Uniprot Accession #P22894). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP8 (neutrophil collagenase) is expressed in neutrophils, where it is stored in specific granules. MMP8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNFalpha and GM-CSF. MMP8 is capable of cleaving types I, II and III triplehelical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, betacasein and peptides such as angiotensin and substance P. In addition to its function in phagocytosis, MMP8 has a high capacity for infiltrating connective tissue, and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP8 consists of several domains: a prodomain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region and a hemopexinlike domain. MMP8 is heavily glycosylated.
Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified human recombinant human MMP-12). The IgG2 fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.
Antigen: recombinant human MMP-8. Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MMP-8 are readily searchable from our website. Different antibodies against the same target such as MMP-8 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.