|The MPO mpo (Catalog #MBS584749) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MPO, Human, mAb 266-6K1, Immuno Assay Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s MPO can be used in a range of immunoassay formats including, but not limited to, Immuno Assay (IA), Western Blot (WB).
WB: Samples were subjected to SDS-PAGE and transferred to PVDF membrane.
The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the MPO mpo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MPO mpo product has the following accession number(s) (GI #4557759) (NCBI Accession #NP_000241.1) (Uniprot Accession #P05164). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
The monoclonal antibody 266-6K1 recognizes human myeloperoxidase (MPO), an ~135 glycoprotein expressed in all cells of the myeloid linage. MPO functions as an alpha2beta2 heteromultimer consisting of two heavy (alpha) and two light (beta) chains of 55 and 15 kDa respectively. MPO is abundantly present in azurophilic granules of polymorphonuclear neutrophils (PMNs). It is an important enzyme used during phagocytic lysis of engulfed foreign particles which takes part in the defense of the organism through production of hypochlorous acid (HOCl), a potent oxidant. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Upon activation of neutrophils, MPO can be rapidly released and as such useful in body fluids as marker for inflammatory status.
Involvement of MPO has been described in numerous diseases such as atherosclerosis, lung cancer, Alzheimer\'s disease, inflammatory bowel disease and multiple sclerosis. Autoimmune antibodies to MPO (so called ANCA) are involved in Wegener\'s disease. Since the discovery of MPO deficiency, initially regarded as rare and restricted to patients suffering from severe infections, MPO has attracted more clinical attention.
Positive Control: Neutrophils, HL-60 cells. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MPO are readily searchable from our website. Different antibodies against the same target such as MPO may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.