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anti-MYD88 antibody product blog

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Posted on 2015-11-29 12:07:38 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; MyD88; anti-MYD88 antibody;
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The MYD88 myd88 (Catalog #MBS200244) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MYD88 antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s MYD88 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS).
Recommended starting dilution is 1:1000. Researchers should empirically determine the suitability of the MYD88 myd88 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The MYD88 myd88 product has the following accession number(s) (GI #197276654) (NCBI Accession #NP_002459) (Uniprot Accession #Q99836). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MYD88 antibody with the following immunoassay(s):
Western Blot (WB) (The cell lysates of Raji (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYD88 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB) MYD88.

Immunofluorescence (IF) (ICC/IF analysis of MYD88 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MYD88 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF) MYD88.

Myeloid differentiation primary response gene 88 (MYD88) is adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. This protein increases IL-8 transcription. It is involved in IL-18-mediated signaling pathway. MYD88 activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes.

Antigen Species: Human
Immunogen: Recombinant human MYD88 (1-309aa) purified from E. coli. Clone Note: Anti-human MYD88 mAb, clone AT22F11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MYD88 protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MYD88 are readily searchable from our website. Different antibodies against the same target such as MYD88 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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