|The MYD88 myd88 (Catalog #MBS249490) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-MYD88 Antibody (clone 1B4) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s MYD88 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS).
Flow Cytometry (1:100)
IHC-P (10 ug/ml)
WB (1:2000). Researchers should empirically determine the suitability of the MYD88 myd88 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MYD88 myd88 product has the following accession number(s) (GI #197276654) (NCBI Accession #NP_002459.2) (Uniprot Accession #Q99836). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-MYD88 Antibody (clone 1B4) IHC-plus with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-MYD88 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunohistochemistry (IHC) (Anti-MYD88 antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Immunofluorescence (IF) (Anti-MYD88 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MYD88.)
Western Blot (WB) (HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYD88 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYD88.)
Flow Cytometry (FC/FACS) (Flow cytometry of HeLa cells, using anti-MYD88 antibody (Red), compared to a nonspecific negative control antibody (Blue).)
Flow Cytometry (FC/FACS) (HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MYD88 antibody, and then analyzed by flow cytometry.)
Flow Cytometry (FC/FACS) (Flow cytometry of Jurkat cells, using anti-MYD88 antibody (Red), compared to a nonspecific negative control antibody (Blue).)
MYD88 Antibody, MYD88D Antibody Description: The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-kB activation.
Immunogen: MYD88 antibody was raised against full length human recombinant protein of human MYD88 (NP_002459) produced in HEK293T cell.
Immunogen Description: Full length human recombinant protein of human MYD88 (NP_002459) produced in HEK293T cell.
Immunogen Type: Recombinant protein.