anti-N/A antibody product blog
Tags: Antibody; Laminin; Monoclonal Antibody; anti-N/A antibody; N/A ;
The LAMC2 lamc2 (Catalog #MBS460282) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Laminin Monoclonal Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Laminin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB).Western Blot: 1-2 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunohistochemistry: 1:50
Immunoprecipitation: 2-5 ug/ml
Optimal dilutions should be determined by researchers for the specific applications. Researchers should empirically determine the suitability of the LAMC2 lamc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The LAMC2 lamc2 product has the following accession number(s) (GI #34232) (NCBI Accession #CAA78729.1) (Uniprot Accession #Q13753). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse anti Laminin Monoclonal Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (IHC: Human lung tissue FFPE slides stained with mouse anti-Laminin monoclonal antibody (MBS460282) at 1:200 for 10 min @ RT, Visualized by using DAB chromogen color system. Note: Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Laminin family consists of 5 subunits,A B1,B2 M and S. Laminin is an ubiquitous non-collagenous connective tissue glycoprotein that is a major constituent of basement membranes. lt is broadly expressed in: skin, heart, lung, and the reproductive tracts. The expression of Iaminin (Ln)alpha1-chain in developing and adult human tissues ln both fetal and adult kidney, a distinct immunoreaclivity was seen in basement membranes (BM). The laminin A chain is present in epithelial, endothelial, and smooth muscle basement membranes. The laminin protein can be phosphorylated in Hela cells by EGF stimulation in vitro.
Antigen Preparation: Purified recombinant partial length of human Laminin protein
Positive Control: Lung
Cellular location: Membrane. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing LAMC2 are readily searchable from our website. Different antibodies against the same target such as LAMC2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.