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anti-NAMPT antibody product blog

Posted on 2018-06-30 17:45:31 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; NAMPT; anti-NAMPT antibody; NAMPT/Visfatin;
The NAMPT nampt (Catalog #MBS2401311) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-NAMPT/Visfatin Antibody (clone OMNI379) reacts with Human
Predicted: Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NAMPT/Visfatin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB).
ELISA: 1:2000-1:10000
FC/FACS: 1:1000
ICC: 1:500-1:1000
IHC-P: 1:500-1:1000, 15 ug/ml
IP: 1:500-1:1000
WB: 1:2000-1:5000. Researchers should empirically determine the suitability of the NAMPT nampt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The NAMPT nampt product has the following accession number(s) (GI #5031977) (NCBI Accession #NP_005737.1) (Uniprot Accession #P43490). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-NAMPT/Visfatin Antibody (clone OMNI379) with the following immunoassay(s):
Immunohistochemistry (IHC) (Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC) NAMPT.

Immunofluorescence (IF) (Immunofluorescent confoacal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Nampt(Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).)
Immunofluorescence (IF) NAMPT.

Western Blot (WB) (Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) at 1: 2, 000 dilution. 1. IBMX. + DEX. - treated 3T3-L1 cell lysate. 2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.)
Western Blot (WB) NAMPT.

Western Blot (WB) (Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with anti-human Nampt(Visfatin/PBEF) pAb.)
Western Blot (WB) NAMPT.

Western Blot (WB) (Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) at 1: 2, 000 dilution. 1. Human Nampt(Visfatin/PBEF) (His-tagged). 2. Human Nampt(Visfatin/PBEF) (FLAG-tagged). 3. Mouse Nampt(Visfatin/PBEF) (His-tagged). 4. Rat Nampt(Visfatin/PBEF) (His-tagged). 5. Human Nampt(Visfatin/PBEF) (His-tagged). 6. LPS-treated human peripheral blood leukocyte lysate.)
Western Blot (WB) NAMPT.

Catalyzes the condensation of nicotinamide with5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limitingcomponent in the mammalian NAD biosynthesis pathway. the secreted formbehaves both as a cytokine with immunomodulating properties and anadipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level inextracellular space and plasma.

Immunogen: Recombinant human Nampt (visfatin/PBEF).
Conjugation: Unconjugated. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NAMPT are readily searchable from our website. Different antibodies against the same target such as NAMPT may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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