|The NANOG nanog (Catalog #MBS850241) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NANOG Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s NANOG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA).
WB: 1/500 - 1/2000, IF: 1/200 - 1/1000, ELISA: Propose dilution 1/10000
Not yet tested in other applications. Determining optimal working dilutions by titration test. Researchers should empirically determine the suitability of the NANOG nanog for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NANOG nanog product has the following accession number(s) (GI #153945816) (NCBI Accession #NP_079141.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NANOG Antibody with the following immunoassay(s):
Testing Data (Figure 1. Western blot analysis using NANOG mouse mAb against NTERA-2 cell lysate (2).)
Testing Data #2 (Figure 2. Confocal immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog mouse mAb (green). Red. Actin filaments have been labeled with DY-554 phalloidin.)
Background: NANOG. Nanog homeobox. Entrez Protein NP_079141. Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression were detected by Northern analysis in the undifferentiated N-Tera embryonal carcinoma cell line.
Immunogen: Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli.
Species: Mouse IgG1. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NANOG are readily searchable from our website. Different antibodies against the same target such as NANOG may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Brain Diseases, Breast Neoplasms, Carcinoma, Squamous Cell, Death, Disease Models, Animal, Head and Neck Neoplasms, Neoplasm Metastasis, Neoplasms, Experimental, Neoplasms, Germ Cell and Embryonal, Nervous System Diseases are some of the diseases may be linked to NANOG Antibody.