|The Nav beta 2 scn2b (Catalog #MBS807208) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Mouse Nav beta 2 Monoclonal IgG2B reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Nav beta 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF).
Western Blot: 1:1000. Researchers should empirically determine the suitability of the Nav beta 2 scn2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Nav beta 2 scn2b product has the following accession number(s) (GI #67906204) (NCBI Accession #NP_001014761.1) (Uniprot Accession #Q56A07). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Anti-Mouse Nav beta 2 Monoclonal IgG2B with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Rat Brain showing detection of ~40 kDa Nav-Beta-2 protein using Mouse Anti-Nav-Beta-2 Monoclonal Antibody, Clone S395-68 (SMC-485). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Nav-Beta-2 Monoclonal Antibody (SMC-485) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~40 kDa.)
NavBeta2 or Scn2B, plays a crucial role in the assembly, expression and functional modulation of the heterotrimeric complex of the sodium channel. The subunit, beta-2 causes an increase in the plasma membrane surface area and in its folding into microvilli. It also interacts with TNR, playing a crucial role in clustering and regulation of activity of sodium channels at the nodes of Ranvier (1, 2). Mutations in this gene have been linked to Brugada syndrome and atrial fibrillation (3).
Immunogen: Fusion protein amino acids 1-215 (full-length) of mouse NavBeta2
Immunogen Species: Mouse. COA: A 1:100 dilution was sufficient for detection of NavBeta2 in 20 ug of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Storage Buffer: PBS pH7.4, 50% glycerol, 0.1% sodium azide
Cellular Localization: Membrane. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Nav beta 2 are readily searchable from our website. Different antibodies against the same target such as Nav beta 2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.