|The Nav beta 3 scn3b (Catalog #MBS807046) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Rat Nav beta 3 Monoclonal IgG2B reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Nav beta 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF).
Western Blot: 1:1000. Researchers should empirically determine the suitability of the Nav beta 3 scn3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Nav beta 3 scn3b product has the following accession number(s) (GI #20806175) (NCBI Accession #NP_620797.1) (Uniprot Accession #Q9JK00). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Anti-Rat Nav beta 3 Monoclonal IgG2B with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Mouse Brain showing detection of ~40 kDa Nav Beta 3 protein using Mouse Anti-Nav Beta 3 Monoclonal Antibody, Clone S396-29. Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Primary Antibody: Mouse Anti-Nav Beta 3 Monoclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~40 kDa.)
Nav Beta 3 (SCN3B) belongs to the voltage-gated sodium channel group, and modulates channel gating kinetics. It inactivates the sodium channel opening more slowly than its Beta1 subunit. It is also unique in causing persistent sodium currents which are thought to amplify summation of synaptic imputs which is likely to increase the excitability of specific neurons to their individual inputs (2). Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons (1, 2). Defects in SCN3B are the cause of Brugada syndrome (3).
Immunogen: Fusion protein amino acids 1-215 (full-length) of rat NavBeta3
Immunogen Species: Rat. COA: A 1:100 dilution was sufficient for detection of NavBeta3 in 20 ug of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Storage Buffer: PBS pH7.4, 50% glycerol, 0.1% sodium azide
Cellular Localization: Membrane. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Nav beta 3 are readily searchable from our website. Different antibodies against the same target such as Nav beta 3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.